Sep 15, 2016 among invertebrate fungal pathogens, beauveria bassiana has assumed a key role in management of numerous arthropod agricultural, veterinary and forestry pests. Pdf beauveria bassiana balsamo vuellemin and metarhizium anisopliae metschnikoff sorokin are entomopathogenic fungi epf that. For the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass produced. The present experiments were conducted to find out morphological variation, growth patterns and suitable media for its mass production. Influence of entomopathogenic fungus beauveria bassiana on. Beauveria bassiana an overview sciencedirect topics. Beauveria is typically deployed in one or more inundative applications of large numbers of aerial conidia in dry or liquid formulations, in a chemical paradigm. Gopalakrishnan, 1999 reported that sorghum was the ideal cereal for the mass production of paecilomyces farinosus. Liquid culture fermentation for rapid production of.
Mass production is mainly practiced by solidstate fermentation to yield. The ability to convert to the yeastlike phase may be a prerequisite for pathogenicity fig. Whey is inexpensive, available in bulk, and its use will provide an alternative outlet for cheese producers. Two isolates of fungal entomopathogen beauveria bassiana balsamo vuillemin hypocreales. Beauveria bassiana is a fungus and gha is a specific strain of that fungus. Different concentrations of spent wash, a waste product from sugar factories, tested for the growth and spore production ofb. For mass production of entomopathogenic fungus beauveria bassiana 149, isolated from moth larva, by. Mass production of beauveria bassiana white muscardine fungus. In this experiment, conidia production of insect pathogenic fungi b. Reports published on relevant research from belgium, canada, china, cuba, czechoslovakia former, france, germany, great britain, philippines, poland, switzerland, usa and ussr former regarding this.
Rice, wheat, maize, sorghum, mini potato tubers, rice bran and rice straw were evaluated for mass production of three beauveria bassiana balsamo vuillemin strains. Mass production of beauveria bassiana jeong jun kim, ji hee han and sangyeob lee agricultural microbiology division, national academy of agricultural science, rda, jeonju 565851, korea abstract. Pdf mass production of entomopathogenic fungi using. Mar 21, 2008 two isolates of fungal entomopathogen beauveria bassiana balsamo vuillemin hypocreales. Suitability of agricultural byproducts as production.
Influence of entomopathogenic fungus beauveria bassiana. Conidial production of beauveria bassiana on agricultural. Mass production of entomopathogenic fungi beauveria bassiana balsamo by using agricultural products based on liquidsolid diphasic method for date palm pest control. In the present study, mass production of metarhizium anisopliae was carried out in the laboratory by using liquid and solid media. Pdf the production and uses of beauveria bassiana as a. Lubilosa system which is particularly well adapted to the production of epfs b. Amongst the solid media rice husk in addition of 2% dextrose was superior to others in terms of spore production 6. Production of beauveria bassiana fungal spores on rice to. Investigations were carried out towards the mass production of entomopathogenic fungi anisopliae substrates, production 3.
Table 2 liquid culture production of biomass and blastospores by various isolates of beauveria bassiana grown in 100 ml liquid medium supplemented with basal salts, 100 g l 1 glucose, and 25 g l 1 acid hydrolyzed casein hycaseo or cottonseed. Research article open access screening of liquid media and. Mass production of conidia polyethylene cushions used for a cultivation of other microorganisms were tested as stationary fermenters for a mass production of beauveria bassiana. Beauveria bassiana, entomopathogenic fungi, fungal mass production, biocontrol formulations, fungal storage and stability, microbial control. Rubin, in handbook of pesticide toxicology second edition, 2001. Whey for mass production of beauveria bassiana and metarhizium anisopliae. Beauveria bassiana strain gha is a fungus that is used as a pesticide for controlling many kinds of insects. The active ingredient can be used on all food crops and many nonfood crops at various outdoor and indoor sites. In this study, fungi, the most appropriate medium for the production of beauveria bassiana in liquid and solid phases to produce clamidospore and conidiosopre were elected. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 3% sucrose and 1%. Entomopathogenic fungi beauveria bassiana has the potential to infect a large number of arthropods.
It is important to appreciate the effect of entomopathogenic fungi on the. The principal objective of this study was to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of beauveria bassiana. Reports published on relevant research from belgium, canada, china, cuba, czechoslovakia former, france, germany, great britain, philippines, poland, switzerland, usa and ussr former regarding this topic. Fungal allergens constitute a significant proportion of the airborne allergens. Effect of growing media and water volume on conidial. Mechanisms involved in the entomopathogenesis of beauveria bassiana death of the host, fungal growth reverts back to the typical hyphal form the saprotrophic stage. Combined effect of entomopathogenic fungus beauveria. Conditions to optimize mass production of metarhizium. Among invertebrate fungal pathogens, beauveria bassiana has assumed a key role in management of numerous arthropod agricultural, veterinary and forestry pests. The objective of this study was to optimize the mass production conditions of m. Mass production of entomopathogenic fungi metarhizium. The optimal initial ph for the spore production of b. The toxic effect of fungus on offspring production a. Beauveria bassiana is an entomopathogenic fungus currently under development as a biological control agent against a wide rang of arthropod pests.
A simple liquid medium which enhanced the production of conidiospores by an isolate of the entomophagous fungus beauveria bassiana is described. Residues of the pesticide are not expected to remain on treated food or feed. The mechanical and manual harvesting methodologies used had low efficiency to extract the spores from the rice grain and this result combined with the low spore productivity less than 1. Mass production of entomopathogenic fungi beauveria. Rice was the best substrate for mass production of b.
Potato broth, rice gruel, coconut water, rice husk, sawdust and rice bran were evaluated for mass culturing of beauveria bassiana bals. Selection of carbon, nitrogen source and carrier for mass. Mass production of beauveria bassiana for regulation of. The organism produces a number of cyclodepsipeptides such as beauvericin which may account for at least part of its insect toxicity miller et al. A novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic beauveria bassiana isolate atp02. The spore count, radial growth, sporulation and biomass were maximum when m. Mass production is mainly practiced by solidstate fermentation. According to ibrahim and low 1993 and sharma, 2002, rice was found to be the suitable media for the mass culture of b. Spore yield and viability were determined for two b. Key words beauveria bassiana, metarhizium anisopliae, entomopathogenic fungi, mass production, diphasic. Biopesticides fact sheet for beauveria bassiana gha. Introduction the global consensus to reduce inputs of chemical. For the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be massproduced. Hence, these two media can be used for successful mass production of fungus, m.
The production and uses of beauveria bassiana as a. The goal of the research herein was to evaluate the use of whey for the mass production of beauveria bassiana and metarhizium anisopliae spores in three wheybased production processes. Mass production and economics of entomopathogenic fungus. Institute of microbiology,hunan province,changsha 49. Among the various concentrations of sugarcane molasses tested, three to six per cent molasses was found to be highly suitable for the radial growth, biomass production and spore production ofbeauveria bassiana. Keywords beauveria bassiana solid state fermentation. Beauveria bassiana, metarhizium anisopliae, verticillium lecanii, mass production, economics. Beauveria bassiana gha is a naturally occuring fungus that is commonly found in soil throughout the world.
May 29, 2014 a novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic beauveria bassiana isolate atp02. The results from this investigation are expected to pave the way towards commercialization ofb. Beauveria bassiana balsamo vuellemin, metarhizium anisopliae metschnikoff sorokin and vertillium lecanii are entomopathogenic fungi epf that occur naturally in the environment and have a potential as biological control agents against many insect pests. Key words beauveria bassiana, metarhizium anisopliae, entomopathogenic fungi, mass production, diphasic liquidsolid fermentation, solid substrates, rice. Sesiidae to beauveria bassiana and metarhizium brunneum.
Potato dextrose agar, saboured dextrose agar, saboured dextrose yeast agar, malt extract agar, czepeck dox agar media were evaluated for mass production of the beauveria bassiana balsamo. Pdf mass production of entomopathogenic fungi beauveria. Strain selection of a fungal entomopathogen, beauveria. Mass production technique of different fungus mass production of green muscardine fungus metarhizium anisopliae mass production of white muscardine fungus beauvaria bassiana. For the liquid phase of plant foods, including potatoes, wheat flour, rice flour, corn flour and sugar cane molasses, and for the solid phase of plant materials, including sugar cane, corn, barley, rice, millet and sorghum. Effect of growing media and water volume on conidial production of beauveria bassiana and metarhizium anisopliae. The cultures were dried and significant differences were found for isolates and time of harvest. Pdf whey for mass production of beauveria bassiana and. Research article open access screening of liquid media. For mass production of entomopathogenic fungus beauveria bassiana 149, isolated from moth larva, by twophase. Biopesticides fact sheet for beauveria bassiana hf23. Biological control, entomopathogenic fungi, mass production. Mass production of beauveria as well as the other fungal entomopathogens is required by the typical inundative use strategy, requiring a costeffective production and. The spores were harvested manually and mechanically and after the.
Mechanisms involved in the entomopathogenesis of beauveria. Pdf entomopathogenic fungi beauveria bassiana has the potential to infect a large number of arthropods. Beauveria bassiana hf23 090305 fact sheet opp chemical code. Here, we provide an overview of massproduction and formulation strategies, updated list of registered commercial products, major biocontrol programs and ecological aspects affecting the use of beauveria as a mycoinsecticide. Clavicipitaceae were grown on cooked rice using diphasic liquidsolid fermentation in plastic bags to produce and harvest spore powder. A laboratory study was conducted to mass produce entomopathogenic fungus, beauveria bassiana, metarhizium anisopliae and verticillium. The epa believes that using this fungus as a pesticide is not expected to have adverse effects on people or the environment and allows its use on food crops. These fungi kill insects by growing on the outer surface and into the bodies of the pests they infect. Aug 22, 2019 sesiidae to beauveria bassiana and metarhizium brunneum. Production of blastospore of entomopathogenic beauveria.
Beauveria bassiana, quercetinase, quercetin2,3dioxygenase, genetic diversity. Introduction beauveria bassiana is a cosmopolitan, soilborn, and the. Table 1 revealed that dry weight of pantnagar isolate of b. Beauveria bassiana isolates used in mass production and pathogenicity trials on eastern spruce budworm choristoneura fumiferana 18 inoculum concentrations for preparatory bioassays of beauveria bassiana against choristoneura fumiferana 21 bioassay design for testing beauveria bassiana against 6th instar choristoneura fumiferana 36. Evaluation of metarhizium anisopliae, beauveria bassiana and paecilomyces fumosoroseus as entomopathogens of the cactus moth, cactoblastis cactorum lepidoptera. Present results provide useful information for developing a simple mass production tactic for targeting sunn pest in their overwintering sites. The spores were harvested manually and mechanically. Studies on the entomopathogenic fungus beauveria bassiana. The highest cost of spore production was recorded in sugarcane bagasse rs. Spore production of beauveria bassiana and metarhizium anisopliae was studied in a novel wheybased culture media. Mass production of endomopathogenic fungus metarhizium. Whey for mass production of beauveria bassiana and.
Beauveria bassiana, a deuteromycete long known for its entomopathogenic properties, causes an insect disease known as white muscardine. Instead, cultivation on solid substrata is required for optimal yields, thus alternative technologies for the massproduction of microorganisms as biocontrol agents. Production of beauveria bassiana air conidia by means of. Conidial production was optimized by the response surface methodology rsm. Objective a field experiment was conducted to investigate the combined effect. White rice and pearled barley mass cultured beauveria. This cereal was also used for the mass production of other deuteromycete fungi.233 555 159 1058 1179 711 697 883 675 791 1081 323 1373 618 1556 71 1221 903 170 923 403 1445 1228 564 1293 1018 1005 939 492 198 748 100 1197 1211 175 733 481 289 1459 93 36 1417